Control of Membrane Permeability in Air-Stable Droplet Interface Bilayers
نویسندگان
چکیده
منابع مشابه
Control of membrane permeability in air-stable droplet interface bilayers.
Air-stable droplet interface bilayers (airDIBs) on oil-infused surfaces are versatile model membranes for synthetic biology applications, including biosensing of airborne species. However, airDIBs are subject to evaporation, which can, over time, destabilize them and reduce their useful lifetime compared to traditional DIBs that are fully submerged in oil. Here, we show that the lifetimes of ai...
متن کاملAir-stable droplet interface bilayers on oil-infused surfaces.
Droplet interface bilayers are versatile model membranes useful for synthetic biology and biosensing; however, to date they have always been confined to fluid reservoirs. Here, we demonstrate that when two or more water droplets collide on an oil-infused substrate, they exhibit noncoalescence due to the formation of a thin oil film that gets squeezed between the droplets from the bottom up. We ...
متن کاملMicrofluidic passive permeability assay using nanoliter droplet interface lipid bilayers.
Membrane permeability assays play an important role in assessing drug transport activities across biological membranes. However, in conventional parallel artificial membrane permeability assays (PAMPA), the membrane model used is dissimilar to biological membranes physically and chemically. Here, we describe a microfluidic passive permeability assay using droplet interface bilayers (DIBs). In a...
متن کاملDynamic morphologies of microscale droplet interface bilayers.
Droplet interface bilayers (DIBs) are a powerful platform for studying the dynamics of synthetic cellular membranes; however, very little has been done to exploit the unique dynamical features of DIBs. Here, we generate microscale droplet interface bilayers (μDIBs) by bringing together femtoliter-volume water droplets in a microfluidic oil channel, and characterize morphological changes of the ...
متن کاملEngineering plant membranes using droplet interface bilayers
Droplet interface bilayers (DIBs) have become widely recognised as a robust platform for constructing model membranes and are emerging as a key technology for the bottom-up assembly of synthetic cell-like and tissue-like structures. DIBs are formed when lipid-monolayer coated water droplets are brought together inside a well of oil, which is excluded from the interface as the DIB forms. The uni...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Langmuir
سال: 2015
ISSN: 0743-7463,1520-5827
DOI: 10.1021/la504712g